Picometrics Technologies is an analytical instrumentation company, which manufactures and sells a range of LIF (Laser Induced Fluorescence) detectors and LEDIF (LED Induced Fluorescence) detectors. Based in Toulouse, France, the Company has established a long standing reputation in high-precision fluorescence detection by employing a unique technology incorporating a number of innovative developments. The detector is commonly employed in R&D and quality control laboratories in pharmaceutical companies, research institutes and universities in Europe, North America and Asia.
The LIF and LEDIF detectors are coupled to High Performance Liquid Chromatography (HPLC – fast-LC, capillary LC and nano LC) and Capillary Electrophoresis (CE) separation systems, to determine the concentration of a compound in a complex micro sample. The detectors are capable of detecting exceedingly small levels, typically nano molar (10-9) and pico molar (10-12) of the compound of interest.
Picometrics provides the market with a unique offer of detectors covering a large range of wavelengths from UV to visible to quantify naturally fluorescent compounds as well as derivatives. The company can assist the analysts in the development of separation methods as well as derivatization methods.
The detectors are employed in a broad range of applications including the analysis of Amino acids, Homocystein and Cystein, Peptides, Biogenic amines, Proteins/Oligoproteins, Sugars, Alcohols/Thiols, Organic acids, Pesticides and Aromatic Pollutants.
Patented Collinear Optical Arrangement
Picometrics offers a unique patented collinear optical arrangement making the detector performances unsurpassed.
The key feature of this patented technology is the use of a ball lens in direct contact with a capillary or micro- column, enabling both high fluorescence recovery and ease-of -use. Fluorescence is read directly inside the capillary, without degrading the resolution of the analytical chain. No optical fine tuning is required when changing the capillary.
Moreover, recent upgrade of this unique technology allowed a superb miniaturization of the optical head, linked to the light source and detector by optical fibers. This design makes easy the integration of the detector in any analytical system.
Laser Induced Fluorescence (LIF) Detector
Picometrics Zetalif Laser induced fluorescence (LIF) detectors are ultra sensitive solutions for all separation techniques such as Liquid Chromatography (capillary LC, microLC, nanoLC, UPLC) and Capillary Electrophoresis. The modular design of this detector provides maximum versatility and enables an unrivalled level of sensitivity for fluorescence measurement thanks to a broad range of lasers offering an excitation wavelength from 266nm to 785nm. Analysts can adapt the wavelength of the laser depending on their application, simply by changing the laser and the corresponding filter blocks.
LED Induce Fluorescence (LEDIF) Detector
The Zetalif LED induced fluorescence (LEDIF) detectors are sensitive solutions for all separation techniques such as Liquid Chromatography (capillary LC, microLC, nanoLC, UPLC) and Capillary Electrophoresis. A large range of LED wavelengths is available: 365, 450, 480, 530 and 640 nm. Picometrics developed this LED based detector to propose the analysts almost equal sensitivity as laser technology with a significantly reduced cost.
LED offers practical advantages:
- The LED is completely integrated inside the detector casing
- Lifetime is very long (>20000 hours) and cost is reduced compared to lasers
- Energy consumption is reduced too, less than 15W
Wavelength and Applications
Zetalif LED detectors offer a wide range of LEDs and covering various applications fields:
- 365 nm for mono and polysaccharides
- 450 nm for amino acids, mono and polysaccharides
- 480 nm for amino acids, DNA/RNA, peptides, oligonucleotides
- 530 nm for immunoglobulins, IgG, oligonucleotides
- 640 nm for oligonucleotides
Analysis of Catecholamines labeled with NBD-F